Chemistry Laboratory Report
Paper Chromatography
Data analysis
From the experimental investigation, the data which were primarily collected from the experiment comprised of the spot distance and the experimentally obtained solvent distance. The obtained data are then effectively used in carrying out the calculations for the R results that were obtained from the experiment (Block et al.). The obtained experimental results show the clear relationship that exists between the known amino acids and the unknown mixtures that were used in the experiment(Viejo 259). Analysis of one of the mixture colors, the unknown mixture that was noted at spot five, the same purple color. From the experimental findings, it is apparent that only one of the two unknown mixtures that were used in the experiment was arginine. This finding was obtained on the basis of RF results and color (Block et al.). Conclusions can, therefore, be made from the experimental findings that the unknown mixture that was employed in the paper chromatography experiment comprised of arginine amino acid components (Viejo 261). The second unknown that was obtained at spot 5 did not correspond to any known amino acid that was used in the experiment. No properties can be drawn for the second component at spot 5. The component drew some identity to glycerin. It cannot, however, be concluded that it was glycine because of the disparities in terms of the retention and the variation in color.
Conclusion
The paper chromatography experimental findings reiterate the importance of paper chromatography in terms of helping in the identification of unknown amino acids and the analysis of relevant mixtures which are characterized by properties that are easily separated by the chromatography paper. During the separation process, it was clear that the cohesion and the adhesion theory play a critical role in the separation process
Works Cited
Block, Richard J., Emmett L. Durrum, and Gunter Zweig. A manual of paper chromatography and paper electrophoresis. Elsevier, 2016.
Viejo, Jacques P. “A Simple Theoretical Approach of Aqueous Solutions of Salts and Organic Solvents in Paper Chromatography.” American Journal of Analytical Chemistry 10.8 (2019): 255-285.