Material and Method:
The experimental study was carried out at Al-Tibri Medical College and Hospital, six months from October 2018 to November 2019. After taking ethical consideration from the ethical review committee of the concerned institute and the animals, they were taken from the animal house of Al-Tibri Medical College and Hospital. A total of 48 male Wister albino rats were taken through a randomized sampling technique with a weight of 150-250gms and equally divided into four different groups and kept them in separate cages for six weeks. Group A (control group) given standard diet and1 unit normal saline intraperitoneal daily once a day, Group B (Experimental group) given inj. Phenytoin 10mg/kg/body wt intra-peritoneal once daily. Group C (Virgin coconut oil) 6.7ml orally and the same dose of phenytoin once daily intra-peritoneal. Group D received 2.5 ml once daily, along with a similar application of inj. Phenytoin. The entire treatment plan was given for six weeks, and the sample was taken on the 4th, 5th, and 6th weeks. Throughout the period, 12hours of day and light cycle were maintained.
Sampling:
Before starting the study, the weight of the animals was divided into four groups. At 4th, 5th and 6th week of the study, weight of animals were taken, and the animals were anesthetized with ethanol containing jar. After given anesthesia, the animals were sacrificed, and through dissection, the testis was removed and stored in formalin, the tissue of the testis were sent for preservation, embedding, and staining process for the histomorphological examination of the tissue. The tissue was stained with H&E.
Histomorphology:
The tubular dimension was taken from different field areas of the slide (choose five field area). The tubular diameter was measured through micrometer, and then the mean of five different field areas at 400x were taken for the comparison among different groups. The reading was recorded in um.
Tubular Dimension= length x Breath/2
Photomicrograph was taken from DSLR camera for the comparison among the different groups.
Statistical Analysis:
The Mean diameter of seminiferous tubules was recorded, and to compare the mean difference among the groups, the one way ANOVA followed by post hoc tukey’s was applied. The level of significance was considered p=<0.05.